| Protein name |
Chymosin B |
| Host compatibility |
Kluyveromyces lactis |
| Yield |
Not reported in g/L, 28 000 SU/ml (Zheng et al., 2025) **
|
| Scale (in specific example) |
Pilot scale (Zheng et al., 2025)
|
| Biggest scale product has been develloped in this organism |
Industrial (DSM) |
| Regulatory status in Europe |
EFSA approved chymosin from the the GM K. lactis strain used by DSM, but it is not allowed for the use in food yet. (Lambré et al., 2022a)
|
| Regulatory status in other parts of the world |
FDA GRAS approval in the US. Not allowed in Canada
|
| Companies producing this protein in this organism |
DSM
|
| Extra/remark |
|
| Publications/references |
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Zheng, Y., Wang, S., Deng, Y., Hu, P., Xue, Q., Li, J., Lei, L., Chan, Z., Yang, J., & Peng, W. (2025). Enhanced production of recombinant calf chymosin in Kluyveromyces lactis via CRISPR-Cas9 engineering. Bioresource Technology, 132116. https://doi.org/10.1016/j.biortech.2025.132116
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Lambré, C., Baviera, J. M. B., Bolognesi, C., Cocconcelli, P. S., Crebelli, R., Gott, D. M., Grob, K., Lampi, E., Mengelers, M., Mortensen, A., Rivière, G., Steffensen, I., Tlustos, C., Van Loveren, H., Vernis, L., Zorn, H., Aguilera, J., Andryszkiewicz, M., Di Piazza, G., . . . Chesson, A. (2022a). Safety evaluation of the food enzyme chymosin from the genetically modified Kluyveromyces lactis strain CHY. EFSA Journal, 20(8). https://doi.org/10.2903/j.efsa.2022.7462
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Barros, R. F., De Marco, J. L., Piva, L. C., Coelho, C. M., De Moraes, L. M. P., & Torres, F. A. (2025). A comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii. Journal of Dairy Science. https://doi.org/10.3168/jds.2025-26935
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Chitpinityol, S., & Crabbe, M. C. (1998). Chymosin and aspartic proteinases. Food Chemistry, 61(4), 395–418. https://doi.org/10.1016/s0308-8146(97)00090-3
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Hayes, M. G., Oliveira, J. C., McSWEENEY, P. L. H., & Kelly, A. L. (2002). Thermal inactivation of chymosin during cheese manufacture. Journal of Dairy Research, 69(2), 269–279. https://doi.org/10.1017/s0022029902005472
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Vallejo, J., Ageitos, J., Poza, M., & Villa, T. (2012). Short communication: A comparative analysis of recombinant chymosins. Journal of Dairy Science, 95(2), 609–613. https://doi.org/10.3168/jds.2011-4445
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Fährenmark, J., Lagerlund, I., Morenweiser, R., Caussette, M., Piron, E., & Ab, A. (2002, June 27). WO2002050253A1 - A method for the purification of chymosin - Google Patents. Retrieved September 4, 2025, from https://patents.google.com/patent/WO2002050253A1/enI
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